beMatrix®
Unlocking the potential of purified collagen and gelatin ingredients for next-generation biomedical applicationsbeMatrix® Biomedical Solutions
Our beMatrix® gelatin and collagen products are created using proprietary technology to yield unmatched safety and quality. Controlled for exceptionally low endotoxin levels and enhanced purity, beMatrix® products are well suited for a range of biomedical applications including cell sheet transportation, cell transplantation, bone void fillers, and more.
Uniquely Suited to Biomedical Applications
Biocompatibility & Biodegradability
Derived from animal tissues, collagen and gelatin demonstrate excellent biocompatibility and biodegradability. In addition, each of these components offers unique functionality, useful for biomedical applications including research and devices.
Materials that can be easily processed into various shapes are needed in the quickly-evolving medical field, especially for regenerative medicine. Both collagen and gelatin have exceptional formability, and have been applied to various uses including bone grafts, artificial skin, DDS carrier, etc. Recently, innovative techniques including 3D printing and electrospinning have been employed to achieve a new degree of control over the shape and formability of systems containing collagen and gelatin.
Valuable Functional Characteristics
Most cells express collagen binding proteins (e.g. Integrin) on their surface; therefore, collagen shows impressive cell attachment.
Some types of cells can efficiently grow and differentiate by recognizing collagen molecules. Taking advantage of this property, collagen is being used as a superior raw material in medical devices including artificial skin and bone grafts. It has also been successfully utilized as a culture substrate for tissue formation from stem cells.
Collagen solutions can readily form gels by adjusting the salt concentration to physiological conditions and incubating at 37°C. Therefore, it is possible to culture cells in or on collagen gel. Collagen gel has established itself as a valuable research tool, and has recently been applied to medical uses including transplantation and as a carrier for cell therapy.
It is known that the cell adhesiveness of gelatin is not strong. However, gelatin can strongly bond with fibronectin, which is a cell adhesion molecule existing in blood or in culture medium. Therefore, it is possible to achieve high cell adhesion in culture devices by gelatin coating.
Gelatin solution has sol-gel transition type-temperature responsivity. Thus, it can form a gel on cooling and can be liquefied upon heating. This thermoreversible reaction is the most unique function of gelatin and is used for various applications.
Gelatin can readily dissolve in water and some organic solvents. Its several amino and carboxyl groups can be easily modified with various chemical reagents.
Some types of gelatin show high viscosity, making it possible to alter the viscosity of medical drugs to achieve optimal effects gelatin incorporation.
Gelatin is a hydrophilic colloid displaying a protective colloidal property. It can be used to successfully inhibit the flocculation of hydrophobic colloids.
beMatrix® Low Endotoxin Gelatin
Recently, highly purified gelatin-based materials have proven valuable to the field of regenerative therapy. In response to customer demands, Nitta has developed beMatrix® gelatin to meet the highest safety and quality requirements of this growing market.

Gelatin LS-250
Sterilized alkaline-treated gelatin derived from porcine skin with high gel strength.
- Low endotoxin level: NMT 10 EU/g (USP)
- Storage condition: Room temperature
- Best before: 3 yrs from date of manufacture

Gelatin LS-H
Alkaline-treated gelatin derived from porcine skin with high gel strength.
- Low endotoxin level: NMT 10 EU/g (USP)
- Storage condition: Room temperature
- Best before: 3 yrs from date of manufacture

Gelatin LS-W
Alkaline-treated gelatin derived from porcine skin with low gel strength.
- Low endotoxin level: NMT 10 EU/g (USP)
- Storage condition: Room temperature
- Best before: 3 yrs from date of manufacture
Safety Testing
Rigorous safety testing confirms the above products to be negative for the five parameters listed below, in comparison to respective controls.
- cytotoxicity
- Sensitization
- Intradermal reaction
- pyrogen
- antigenicity


Gelatin HG
Sterilized gelatin hydrolysate derived from porcine skin, enhanced in safety and non-gelling. Tested for acute systemic toxicity and subacute toxicity, in addition to 5 safety test parameters listed above
- Low endotoxin level: NMT 10 EU/g (USP)
- Storage condition: Room temperature
- Best before: 3 yrs from date of manufacture
beMatrix® Low Endotoxin Collagen
beMatrix® collagen was developed in response to the demands of the growing regenerative medical market. This unique product line includes a variety of collagen formats with reduced endotoxin and virus levels.
beMatrix® collagen AT was used as the culture material for retinal pigment epithelial cell sheets in the first ever clinical study on iPS cell-derived tissue transplantation, conducted by Dr. Masayo Takahashi.
Collagen At
Acid extracted collagen solution from porcine tendon.
- Low endotoxin level: NMT 0.5 EU/ml
- Storage condition: Refrigerated storage (4 to 8 °C)
- Best before: 2 yrs from date of manufacture
- Concentration: 3 mg/ml [pH 3]

Collagen te
Pepsin-solubilized collagen solution from porcine skin.
- Low endotoxin level: NMT 0.5 EU/ml
- Storage condition: Refrigerated storage (4 to 8 °C)
- Best before: 2 yrs from date of manufacture
- Concentration: 5 mg/ml [pH 3]

Collagen fd
Freeze-dried atelocollagen derived from porcine skin. A virus-inactivated product with high water solubility.
- Low endotoxin level: NMT 100 EU/g
- Storage condition: Room temperature
- Best before: 2 yrs from date of manufacture
- Product appearance: Spongiform
- Size: approx.140×100 x10 mm
